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| 产品名称： | pSLH16 transgene 2 |
|---|---|
| 商品货号： | TS170733 |
| Designations： | pSLH16 transgene 2 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein low density lipoprotein receptor (familial hypercholesterolemia) low density lipoprotein receptor |
| Vector： | Construct size (kb): 17.29998970031738 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pTZ18R Intact vector size: 2.900 Type of vector: phagemid Vector end: NotI Vector end: NotI Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Construction: pUC18, Escherichia coli lacZalpha, pBR322, f1 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: LDLR Genomic copy number: unique Gene name: low density lipoprotein receptor (familial hypercholesterolemia) Contains complete coding sequence?: U Chromosome: 19; Localization: 19 p13.2 Type of DNA: genomic Insert end: NotI Insert end: NotI Insert size (kb): 14.4 Cross references: Insert lengths(kb): 14.39999961853027 Gene product: low density lipoprotein receptor (familial hypercholesterolemia)(low density lipoprotein receptor) LDLR Alleles: A2, E1, E2, A1, A2, B1, B2, D1, D2, K1, K2, Q1, Q2, S2, A1, S1 Target Gene: low density lipoprotein receptor (familial hypercholesterolemia) |
| Insert Size (kb)： | 14.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--14.8, 2.5; EcoRI--12.8, 4.5; BglII--14.0, 3.3; SstI--17.3; NotI--14.4; 2.9. The insert includes the following restriction sites: SalI (intron 1, 2.0 kb from the 5 NotI site); BglII (intron 3); EcoRI (cDNA segment). Encodes the normal sequence. The expected size of the correctly processed mRNA is 2.9 kb. The NotI fragment consists of the following elements: the mouse metallothionein promoter I (1.7 kb); exon 1 of LDLR, containing a SstII site; intron 1 truncated by deletion of a RsaI (77 nt 3 to exon 1) / EcoRI (3.1 kb 5 to exon 2) fragment; exons and introns 2-4; a cDNA fragment corresponding to exons 5-18; and transcription termination and polyadenylation signals from the human growth hormone receptor gene (0.6 kb). |
| References： | Pathak RK, et al. Tissue-specific sorting of the human LDL receptor in polarized epithelia of transgenic mice. J. Cell Biol. 111: 347-359, 1990. PubMed: 2199454 Masayuki Yokode, personal communication |