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| 产品名称： | pET-His |
|---|---|
| 商品货号： | TS170772 |
| Designations： | pET-His |
| Depositors： | T Hai |
| Biosafety Level： | 1 |
| Host： | E. coli HB101 |
| Vector Information： | Size (kb): 4.636 Vector: pET-His (plasmid) Promoters: Promoter T7 (phi10) Construction: pET3a Marker(s):ampR Construct size (kb): 4.636 Features: marker(s): ampR promoter: T7 (phi10) replicon: pMB1 MCS: NdeI...BamHI terminator: phi10 |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.6; BamHI--4.6; BglI--2.5, 2.1, 0.25. The plasmid contains the following restriction sites (kb from nt 1): BamHI--4.12; BglI--0.87, 3.19, 3.42; BglII--3.99; EcoRI--4.63; HindIII--4.60; NcoI--4.12; NdeI--4.09; PstI--0.75; PvuI--0.62; PvuII--2.29; SalI--3.71; XbaI--4.05; XhoI--4.11. Digestion with NcoI, BamHI or XhoI and subsequent filling of ends, allows cloning of blunt-ended fragments with a +0, +1 (C) or +2 (GA) nucleotide reading frame, respectively. Fusion proteins can be purified using a nickel-chelating column, even under denaturing conditions. Constructed from pET3a by insertion of a double stranded oligonucleotide linker between the NdeI and BamHI sites, creating three restriction sites and encoding six His residues. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Chen BP, Hai T. Expression vectors for affinity purification and radiolabeling of proteins using Escherichia coli as host. Gene 139: 73-75, 1994. PubMed: 8112591 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid |