宁波泰斯拓生物

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Quantitative Synthetic Saint Louis encephalitis virus RNA

货号 TS170895
中文名称 null
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产品简介
购买须知
产品名称: Quantitative Synthetic Saint Louis encephalitis virus RNA
商品货号: TS170895
Agent: Quantitative Synthetic Saint Louis encephalitis virus RNA
Applications: ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format: frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica RNAstable
Storage Conditions: -70°C or colder
Intended Use: The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.

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Comments: Manufactured under ISO 13485 guidance

Preparation includes fragments from pre-membrane, envelope, and nonstructural genes NS1, NS5, and 3’UTR.

The following primers and probe can be used with this nucleic acid preparation RefLanciotti RS, Kerst AG. Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses. J. Clin. Microbiol. 39(12): 4506-4513, 2001. PubMed: 11724870:
Forward primer: CTGGCTGTCGGAGGGATTCT
Reverse primer: TAGGTCAATTGCACATCCCG
Probe: FAM-TCTGGCGACCAGCGTGCAAGCCG-BHQ
Special Collection: RNA
References:

Lanciotti RS, Kerst AG. Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses. J. Clin. Microbiol. 39(12): 4506-4513, 2001. PubMed: 11724870