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微信小章| 产品名称： | p78/2-8b |
|---|---|
| 商品货号： | TS170980 |
| Designations： | p78/2-8b |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.650000095367432 Tissue: embryonic lung HEL cell line, interferon-treated Gene product: myxovirus (influenza) resistance 1, homolog of murine (interferon-inducible protein p78)(myxovirus (influenza) resistance 1, homolog of murine (interferon-inducible protein (p78)Y)) MX Alleles: A1, A2, B1, B2, A1, A2, B1, B2 |
| Insert Size (kb)： | 2.650 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 2.65; MluI--uncut; PstI--3.4, 1.1, 0.9; BamHI--5.6; HindIII--3.1, 2.5. This probe detects the following genomic restriction fragments (kb): NotI--775, BssHII--600; MluI--1225, 1125; NruI--700, SacII--400; SfiI--380. This clone extends from the EcoRI site at position 137 (5 untranslated region) to the 3 end at nucleotide 2788, and thus encodes a complete polypeptide. This clone lacks an alternative exon in the 5 noncoding region (249 to 324) and thus contains a HindIII site at 248. 5 of 10 cDNAs sequenced contain the alternative exon. The polymorphism has been studied using a 1.23 kb PstI fragment from the upstream non-coding and N-terminal coding sequences. In 40 CEPH families, the haplotype frequencies are as follows: A1B1--0.45; A1B2--0.1; A2B1--0.32; A2B2--0.13. There is no significant linkage disequilibrium between the two PstI polymorphisms. |
| References： | Petersen MB, et al. Two PstI DNA polymorphisms adjacent to the human gene for the interferon-induced p78 protein (MX1 gene). Nucleic Acids Res. 17: 7546, 1989. PubMed: 2798118 Horisberger MA, et al. Cloning and sequence analyses of cDNAs for interferon- and virus-induced human Mx proteins reveal that they contain putative guanine nucleotide binding sites: functional study of the corresponding gene promoter. J. Virol. 64: 1171-1181, 1990. PubMed: 2154602 Gardiner K, et al. Analysis of human chromosome 21: correlation of physical and cytogenetic maps; gene and CpG island distributions. EMBO J. 9: 25-34, 1990. PubMed: 2295313 Goetschy JF, et al. Regulation of the interferon-inducible IFI-78K gene, the human equivalent of the murine Mx gene, by interferons, double-stranded RNA, certain cytokines, and viruses. J. Virol. 63: 2616-2622, 1989. PubMed: 2542574 |