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| 产品名称： | pBlueBac/PKC-alpha |
|---|---|
| 商品货号： | TS171004 |
| Designations： | pBlueBac/PKC-alpha |
| GenBank Number： | X52479 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein protein kinase C, alpha |
| Vector： | Construct size (kb): 16.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBlueBac Intact vector size: 14.000 Type of vector: phagemid Vector end: NheI Vector end: NheI Cloning sites: NheI Polylinker sites: Host range: insect cells; Escherichia coli Features (with orientation and position when available): insert detection: lac marker(s): ampR promoter: polyhedrin replicon: pMB1, f1, SV40 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PRKCA Other gene symbols: PKCA Genomic copy number: unique Gene name: protein kinase C, alpha Contains complete coding sequence?: Y Chromosome: 17; Localization: 17 q22-q24 Tissue: liver, HepG2 cell line Type of DNA: cDNA Insert end: SpeI Insert end: SpeI Insert size (kb): 2.0 Cross references: DNA Seq. Acc.: X52479 Insert lengths(kb): 2.0 Tissue: liver, HepG2 cell line Gene product: protein kinase C, alpha PKCA Target Gene: protein kinase C, alpha |
| Insert Size (kb)： | 2.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--10.0, 3.5, 1.3, 1.1, 0.25; BglI--5.8, 5.4, 2.2, 1.6, 1.2; NcoI--16.0; PstI--15.0, 0.65; PvuII--3.3, 2.5 (doublet), 2.3, 1.6, 1.4, 1.2, 0.75, 0.35. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.56, 0.77; BglI--0.94; NcoI--1.15; PstI--0.15; PvuII--1.21, 1.93; SmaI--0.02. Contains the complete coding sequence only (nt 28-2046 of GenBank X52479). The NheI/SpeI sites were destroyed during cloning. |
| References： | Finkenzeller G, et al. Sequence of human protein kinase C alpha. Nucleic Acids Res. 18: 2183, 1990. PubMed: 2336401 David J Burns, personal communication |