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| 产品名称： | pSP65:ClVd#5 |
|---|---|
| 商品货号： | TS171059 |
| Designations： | pSP65:ClVd#5 |
| Species： | Coleus viroid (ClVd) |
| Depositors： | RW Hammond, USDA - ARS |
| Vector： | Name of construct: pSP65:ClVd#5 Excise insert: EcoRI+HindIII Size of construct (kb): 3.2 Markers: ampR Name of vector: pSP65 Intact vector size: 3.010 Type of vector: plasmid Vector end: EcoRI Vector end: HindIII Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Host range: Escherichia coli Features (with orientation and position when available): enhancer: none marker(s): ampR promoter: SP6 replicon: pMB1 terminator: none |
| Insert： | Genome: Coleus viroid (ClVd) Gene name: genome, partial Contains complete coding sequence?: U Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 0.2 |
| Insert Size (kb)： | 0.2 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.1, 0.24; BglI--3.3; HindIII--3.3; PvuII--3.3. A PCR amplification product. |
| Classification： | Coleviroid |
| References： | Rosemarie W Hammond, personal communication |