宁波泰斯拓生物

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pKT1201

货号 TS171159
中文名称 null
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产品简介
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产品名称: pKT1201
商品货号: TS171159
Designations: pKT1201
Species: Rattus norvegicus, rat
Depositors: BR Olsen, R Haronen
Vector:
Construct size (kb): 6.099999904632568
Insert:
DNA: cDNA
Insert lengths(kb): 1.75
Tissue: chondrosarcoma
Gene product: collagen, type XI, alpha 1 COL11A1
Insert Size (kb): 1.75
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): BglI--3.8, 2.4; EcoRI--4.5, 1.6; NdeI--3.8, 2.4.
The insert appears to contain approx. 0.3 kb of linker sequence.
References:

Bernard M, et al. Cloning and sequencing of pro-alpha1(XI) collagen cDNA demonstrates that type XI belongs to the fibrillar class of collagens and reveals that the expression of the gene is not restricted to cartilagenous tissue. J. Biol. Chem. 263: 17159-17166, 1988. PubMed: 3182841

Ritva Haronen, personal communication