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| 产品名称： | cosmid51.22 |
|---|---|
| 商品货号： | TS171198 |
| Designations： | cosmid51.22 |
| GenBank Number： | L01962 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 45.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pWE15 Intact vector size: 8.200 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI Polylinker sites: EcoRI NotI BamHI NotI EcoRI Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR, kanR, G418R promoter: T3, T7 replicon: pMB1 Cross references: DNA Seq. Acc.: X65279 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SCN4A Gene name: sodium channel, voltage-gated, type IV, alpha polypeptide Contains complete coding sequence?: Y Chromosome: 17; Localization: 17 q23.1-q25.3 Tissue: lymphoblast Type of DNA: genomic Insert end: BamHI Insert end: BamHI Insert size (kb): 37.0 Cross references: DNA Seq. Acc.: L01962 DNA Seq. Acc.: L01963 DNA Seq. Acc.: L01964 DNA Seq. Acc.: L01965 DNA Seq. Acc.: L01966 DNA Seq. Acc.: L01967 DNA Seq. Acc.: L01968 DNA Seq. Acc.: L01969 DNA Seq. Acc.: L01970 DNA Seq. Acc.: L01971 DNA Seq. Acc.: L01972 DNA Seq. Acc.: L01973 DNA Seq. Acc.: L01974 DNA Seq. Acc.: L01975 DNA Seq. Acc.: L01976 DNA Seq. Acc.: L01977 DNA Seq. Acc.: L01978 DNA Seq. Acc.: L01979 DNA Seq. Acc.: L01980 DNA Seq. Acc.: L01981 DNA Seq. Acc.: L01982 DNA Seq. Acc.: L01983 Insert lengths(kb): 37.0 Tissue: lymphoblast Gene product: sodium channel, voltage-gated, type IV, alpha polypeptide SCN4A |
| Insert Size (kb)： | 37.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--12.5, 10.5, 5.3, 5.0, 3.8 (doublet), 3.6, 0.8; BglII--15.5 (doublet), 12.0; EcoRI--20.0, 12.0, 8.8, 2.1; HindIII--14.5, 12.0, 7.1, 3.75, 3.6, 2.1; SstI--13.5, 9.4, 5.6, 5.3, 3.4, 1.75, 1.35, 1.05, 0.86. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Extends from exon 1 into exon 24, from the 5 untranslated region into the 3 untranslated region. |
| References： | McClatchey AI, et al. The genomic structure of the human skeletal muscle sodium channel gene. Hum. Mol. Genet. 1: 521-527, 1992. PubMed: 1339144 |