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| 产品名称： | YEp352 |
|---|---|
| 商品货号： | TS171217 |
| Designations： | YEp352 |
| Depositors： | A Tzagoloff |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.18
Vector: YEp352 (plasmid)
Promoters: Promoter lac
Construction: pUC18, 2 micron, URA3
Marker(s): URA3,ampR
Features: insert detection: lacZ
marker(s): ampR, URA3 promoter: lac replicon: pMB1, 2 micron terminator: none enhancer: none |
| Applications： | YE-type (episomal) shuttle vector Shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3. This is a Escherichia coli/yeast episomal shuttle vector with multiple restriction sites for cloning. Constructed by filling in and blunt-end ligating pUC18 linearized at NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI site in URA3 were removed. The yeast genomic sequence is in nt 286-1383 (where the A of the ATG of lacZ is number 1), the 2 micron sequence goes from nt 1384-2768, and pUC18 includes nt 2769-285. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Hill JE, et al. Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2: 163-167, 1986. PubMed: 3333305 Alexander Tzagoloff, personal communication |
| Shipped： | frozen |