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微信小章| 产品名称： | P1 |
|---|---|
| 商品货号： | TS171266 |
| Designations： | P1 |
| GenBank Number： | J04211 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.5 Tissue: placenta Gene product: collagen, type VI, alpha 2 COL6A2 Alleles: b, s, A2, A1 |
| Insert Size (kb)： | 2.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains a BamHI site (in the region encoding the triple-helical domain) and an XhoI site. Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.5; HindIII--5.2; BamHI--3.9, 1.3; PstI--5.2; XbaI--5.2. COL6A1 and COL6A2 are closely linked (about 200 kb). The insert encodes the complete triple-helical domain, extending about 1.5 kb 3 from that domain. The insert detects a mRNA of about 3.5 kb in human fibroblasts. |
| References： | Chu ML, et al. Sequence analysis of alpha1(VI) and alpha2(VI) chains of human type VI collagen reveals internal triplication of globular domains similar to the A domains of von Willebrand facter and two alpha2(VI). EMBO J. 8: 1939-1946, 1989. PubMed: 2551668 Chu ML, et al. Amino acid sequence of the triple-helical domain of human collagen type VI. J. Biol. Chem. 263: 18601-18606, 1988. PubMed: 3198591 Francomano CA, et al. The COL6A1 and COL6A2 genes exist as a gene cluster and detects highly informative DNA polymorphisms in the telomeric region of human chromosome 21q. Hum. Genet. 87: 162-166, 1991. PubMed: 1676701 Justice MJ, et al. A genetic linkage map of mouse chromosome 10: localization of eighteen molecular markers using a single interspecific backcross. Genetics 125: 855-866, 1990. PubMed: 1975791 Mon-Li Chu, personal communication |