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| 产品名称： | p214LT |
|---|---|
| 商品货号： | TS171301 |
| Designations： | p214LT |
| GenBank Number： | J02288 |
| Species： | polyomavirus |
| Depositors： | R Kamen |
| Vector： | Construct size (kb): 5.5 |
| Insert： | DNA: genomic Insert lengths(kb): 2.200000047683716 Gene product: large T antigen |
| Insert Size (kb)： | 2.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze dried Escherichia coli containing the plasmid. |
| Comments： | Constructed from pPyLT-1 by removing the EcoRI fragment. Stop codon lies in plasmid. Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BamHI/BglI--2.2, 1.8, 0.8, 0.6, 0.3; BamHI/EcoRI--3.8, 2.2. The insert contains a truncated portion of the Large T Antigen. |
| References： | Rassoulzadegan M, et al. The roles of individual polyoma virus early proteins in oncogenic transformation. Nature 300: 713-718, 1982. PubMed: 6294529 Robert Kamen, personal communication |