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| 产品名称： | pcDNAI-ALDO4 |
|---|---|
| 商品货号： | TS171302 |
| Designations： | pcDNAI-ALDO4 |
| GenBank Number： | S64136 |
| Species： | Rattus norvegicus, rat |
| Depositors： | CE Gomez-Sanchez, M Zhou |
| Applications： | in another host, produces protein cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase) Cytochrome P450, subfamily XIB, polypeptide 2 (aldosterone synthase), aldosterone synthase, steroid 11-beta/18-hydroxylase, steroid-11-beta-hydroxylase, 11-beta-hydroxylase |
| Vector： | Construct size (kb): 10.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.0 Gene product: cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase)( Cytochrome P450, subfamily XIB, polypeptide 2 (aldosterone synthase), aldosterone synthase, steroid 11-beta/18-hydroxylase, steroid-11-beta-hydroxylase, 11-beta-hydroxylase) CYP11B2 Target Gene: cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase) |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1235: LB Medium (ATCC medium 1065) with 5 mcg/ml tetracycline and 10 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BstXI/NotI--4.4, 2.0, 1.35; BamHI--5.6, 2.3; HindIII--6.6, 1.2; PvuII--2.5, 1.7, 1.6, 0.7, 0.5, 0.3, 0.25; XhoI--5.6, 2.3. The insert contains the following restriction sites (nt from the 5 end): XhoI--848; HindIII--1035; HincII--1257; PvuII--1348, 1957, 2404, 2624; BamHI--2131; BstXI--1233. Contains the complete coding sequence (nt 292-1812). |
| References： | Zhou M, Gomez-Sanchez CE. Cloning and expression of a rat cytochrome P-450 11 beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant. Biochem. Biophys. Res. Commun. 194: 112-117, 1993. PubMed: 8333830 Celso E Gomez-Sanchez, personal communication |