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| 产品名称： | HuVim5 huVIM5 |
|---|---|
| 商品货号： | TS171314 |
| Designations： | HuVim5 huVIM5 |
| Species： | Homo sapiens, human |
| Applications： | Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences. |
| Vector： | Construct size (kb): 8.100000381469727 |
| Insert： | DNA: genomic Insert lengths(kb): 5.0 Gene product: vimentin VIM Alleles: A1, A2 |
| Insert Size (kb)： | 5.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--8.1, BamHI--8.0 + small fragment; EcoRI/BamHI--4.9, 2.7, + small fragment; HindIII--4.1, 3.0, 0.7; SalI--8.1. The insert contains two internal HindIII sites and one BamHI site. Insert contains MspI (5), HindII (2), PstI (5), and HindIII (1) sites. Clone includes exons 3-8, parts of introns 2 and 8, and introns 3-7. The portion of intron 2 includes a repetitive sequence. Exon 6 contains sequence compatible with a hairpin structure. Shows 90% homology with coding region of hamster vimentin. Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences. |
| References： | Lilienbaum A, et al. Vimentin gene: expression in human lymphocytes and in Burkitts lymphoma cells. EMBO J. 5: 2809-2814, 1986. PubMed: 3792301 Perreau J, et al. Nucleotide sequence of the human vimentin gene and regulation of its transcription in tissues and cultured cells. Gene 62: 7-16, 1988. PubMed: 3371665 Denise Paulin, personal communication |