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| 产品名称： | pBP96 |
|---|---|
| 商品货号： | TS171385 |
| Designations： | pBP96 |
| Depositors： | RH Reeves |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.5999999046325680 Vector: pBP96 (plasmid) Construction: BLUR8, HIS3 Marker(s):ampR,HIS3 Construct size (kb): 4.599999904632568 Features: marker(s): ampR, HIS3 replicon: pMB1 |
| Applications： | YI-type (integrating) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 1.6; EcoRI--4.6; PstI--3.3, 1.3. Digestion with BamHI removes the plasmid sequences from the Alu ends for targeting integration. Transformation into pools from a YAC library derived from a human-mouse somatic cell hybrid, and selection for His+, resulted in an approximate 50-fold enrichment for Alu-containing YACs. Larger YACs were preferentially selected. Some YACs demonstrated loss of Alu-containing restriction fragments suggesting internal deletions resulting when the vector targeted two Alu sequences in the YAC. YI-type shuttle integrative vector targeting human Alu sequences in YAC libraries derived from somatic cell hybrids. Constructed by inserting a blunt-ended 1.2 kb fragment containing S. cerevisiae HIS3 into the blunted MscI within the Alu sequence of BLUR8. The order of the major features in this plasmid is: bla - BamHI - Alu - HIS3 - Alu - BamHI. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Pavan WJ, Reeves RH. Integrative selection of human chromosome-specific yeast artificial chromosomes. Proc. Natl. Acad. Sci. USA 88: 7788-7791, 1991. PubMed: 1881915 Reeves RH, et al. Yeast artificial chromosome modification and manipulation. Methods Enzymol. 216: 584-603, 1992. PubMed: 1336105 Roger H Reeves, personal communication |
| Shipped： | freeze-dried |