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| 产品名称： | pGem3-Rat-NFH |
|---|---|
| 商品货号： | TS171422 |
| Designations： | pGem3-Rat-NFH |
| GenBank Number： | AF031879 |
| Species： | Rattus norvegicus, rat |
| Depositors： | RK Liem |
| Vector： | Construct size (kb): 6.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.400000095367432 Tissue: brain, 21-day-old Gene product: neurofilament, heavy polypeptide (200kD)(neurofilament, heavy polypeptide) Nefh |
| Insert Size (kb)： | 3.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.31, 0.80; ClaI--0.39; NcoI--0.47; NotI--0.04; PstI--0.36, 0.70, 0.72, 0.91, 1.02, 1.55; SacI--0.23; XhoI--0.27. Recommendation for verification: EcoRI, SacI, NdeI. |
| References： | Chin SS, Liem RK. Transfected rat high-molecular-weight neurofilament (NF-H) coassembles with vimentin in a predominantly nonphosphorylated form. J. Neurosci. 10: 3714-3724, 1990. PubMed: 2230956 |