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| 产品名称： | pMHZ14 |
|---|---|
| 商品货号： | TS171429 |
| Designations： | pMHZ14 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | HindIII also identifies a 2-allele RFLP (18 kb, 14 kb, with a constant band at 5 kb) but this has not yet been verified with pedigree studies. |
| Vector： | Construct size (kb): 12.0 |
| Insert： | DNA: genomic Insert lengths(kb): 9.0 Gene product: DNA Segment, single copy D1S60 Alleles: A2, A1 |
| Insert Size (kb)： | 9.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | A EcoRI/HindIII double digest reveals fragments of 4.6, 3.8, and 0.4 kb from the insert. Restriction digests of the clone give the following sizes (kb): BamHI--9.0, 3.0; EcoRI/HindIII--4.4, 3.55, 2.85, 0.52; EcoRI--12.0. HindIII also identifies a 2-allele RFLP (18 kb, 14 kb, with a constant band at 5 kb) but this has not yet been verified with pedigree studies. Codominant segregation demonstrated in 52 3-generation families. Enzyme(s) not detecting polymorphism: MspI, RsaI, PvuII, PstI, BglII. |
| References： | Hoff M, et al. Isolation and mapping of a polymorphic DNA sequence pMHZ14 on chromosome 1p (D1S60). Nucleic Acids Res. 15: 9619, 1987. PubMed: 2891119 Yusuke Nakamura, personal communication |