微信小陈
微信小章
产品简介
购买须知
| 产品名称： | HFBCT78 |
|---|---|
| 商品货号： | TS171440 |
| Designations： | HFBCT78 |
| GenBank Number： | T05242 |
| Species： | Homo sapiens, human |
| Depositors： | JC Venter |
| Vector： | Construct size (kb): 3.799999952316284 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PLA2G2A Other gene symbols: PLA2L; PLA2B Gene name: phospholipase A2, group IIA (platelets, synovial fluid) Contains complete coding sequence?: U Chromosome: 1; Localization: 1 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 0.8 Cross references: DNA Seq. Acc.: M86400; EST: EST03131 Nucleotides 1-193 of the insert correspond to nucleotides 2581-2388 of M86400 with 100% identity. DNA Seq. Acc.: T05242; EST: EST03131; Primer: M13-21 Insert lengths(kb): 0.800000011920929 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: phospholipase A2, group IIA (platelets, synovial fluid)(phospholipase A2 polypeptide B (synovial fluid)) PLA2B |
| Insert Size (kb)： | 0.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8. Based on the insert size and the match to the published sequence, probably a partial clone consisting primarily of 3 untranslated region, because the coding sequence of M86400 is nt 85-822. |
| References： | Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434 |