微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pHincIIRM-10-5.7 p(pUC)HincIIRM-10-5.7 |
|---|---|
| 商品货号： | TS171445 |
| Designations： | pHincIIRM-10-5.7 p(pUC)HincIIRM-10-5.7 |
| Depositors： | New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications： | in suitable host, produces protein modification methylase HincII in suitable host, produces protein restriction endonuclease HincII |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 3.0 Gene product: modification methylase HincII hsdM Target Gene: modification methylase HincII, restriction endonuclease HincII |
| Insert Size (kb)： | 3.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.0, 2.7. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--0.1; XbaI--0.6, 1.3; NdeI--0.7; PstI--2.2, 2.4, 2.6; BglII--2.8. The methylase and endonuclease genes are both transcribed in the same direction, with the methylase being 5 to the endonuclease. The methylase gene lies within the first 1.5 kb of the insert; the endonuclease gene within the last 0.9 kb. Distributed in aliquots of 100 ng dried. |
| References： | Rees PA, Brenner JS II. Method for producing the HincII restriction endonuclease and methylase. US Patent 5,015,581 dated May 14 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |