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| 产品名称： | 50f2 |
|---|---|
| 商品货号： | TS171464 |
| Designations： | 50f2 |
| Species： | Homo sapiens, human |
| Depositors： | J Weissenbach |
| Vector： | Construct size (kb): 14.39999961853027 |
| Insert： | DNA: genomic Insert lengths(kb): 1.700000047683716 Gene product: DNA Segment, numerous copies DYS7 |
| Insert Size (kb)： | 1.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.7, 1.7, 1.7, 1.7, 1.7, 1.7; HindIII--3.3, 1.7, 1.7, 1.7, 1.7, 1.7, 1.7; EcoRI/HindIII--3.3, 1.7, 1.7, 1.7, 1.7, 1.7, 1.7; BamHI--14.4; PstI--14.4. EcoRI detects a 2.4 kb autosomal band and 5 Y-specific bands of 10 kb (A, maps to short arm), 7.5 kb (B, short arm), 6 kb (C, long arm), 4.5 kb (D, centromeric) and 1.7 kb (E, long arm). TaqI detects autosomal bands of 11 to 12 kb and 10.5 kb, and 4 Y-specific bands of 9 kb (equivalent to EcoRI band E), 8 kb (EcoRI band D), 3.5 kb (EcoRI band A or B) and 2.8 kb (two fragments, equivalent to EcoRI C and EcoRI A or B). Plasmid contains 6 copies of the 1.7 kb insert. Wash at high stringency (0.1X SSC, 68C). |
| References： | Vergnaud G, et al. A deletion map of the human Y chromosome based on DNA hybridization. Am. J. Hum. Genet. 38: 109-124, 1986. PubMed: 3004206 Jean Weissenbach, personal communication |