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| 产品名称： | pTAG-1 |
|---|---|
| 商品货号： | TS171475 |
| Designations： | pTAG-1 |
| Depositors： | JL Fridovich-Keil |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.0000000000000000 Vector: pTAG-1 (phagemid) Construction: human beta-globin, pGEM-7Zf+, SV40 poly(A), pBLCAT3 polylinker Marker(s):ampR Construct size (kb): 6.0 Features: insert detection: beta-globin marker(s): ampR replicon: pMB1, f1 terminator: SV40 |
| Applications： | promoter-cloning vector reporter construct shuttle vector vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.8, 2.2; SphI--3.8, 2.2; HindIII--6.0; XhoI--6.0; BamHI--5.5, 0.55. A promoter/enhancer-cloning shuttle vector using human beta-globin as the reporter gene. Primarily useful for analysis of gene expression at the RNA level. The ApaI site can be used for cloning enhancer elements. Contains a trimer cassette of the SV40 major late polyadenylation signal to block background readthrough expression of the reporter gene. The order of the major features in this plasmid is: SalI - SV40 polyadenylation trimer - XhoI/MCS/HindIII - beta-globin - PstI - SphI - ApaI - ampR/pGEM-7Zf(+). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Fridovich-Keil JL, et al. Improved expression vectors for eukaryotic promoter/enhancer studies. BioTechniques 11: 572-579, 1991. PubMed: 1725108 |
| Shipped： | freeze-dried |