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| 产品名称： | pIC552 |
|---|---|
| 商品货号： | TS171546 |
| Designations： | pIC552 |
| Depositors： | F Macian, M Armengod |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.3999996185302730 Vector: pIC552 (plasmid) Construction: pIC449, pKK232-8 Marker(s):ampR,spcR,strR Construct size (kb): 9.399999618530273 Features: marker(s): ampR replicon: pMB1 reporter group: galK::lacZ MCS: NcoI...SmaI ribosome-binding site: galK terminator: Omega transcription terminator: rrnB T1 T2 coding sequence: aadA (spcR, strR) |
| Applications： | promoter-cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/SmaI--7.0, 2.3; BamHI/EcoRI--6.55, 2.8; AccI--7.6, 1.8. The omega interposon terminates transcription in both directions. Transcriptional fusions constructed with this vector can be moved to the bacterial chromosome by using the transducing phage lambdaRZ5. This reduces problems inherent with multicopy plasmid systems like gene-dosage and titration effects. Promoter probe vector based on transcriptional fusion to lacZ. Constant efficiency of translation of lacZ is engineere d by incorporation of galK translational start signals. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Macian F, et al. An improved vector system for constructing transcriptional lacZ fusions: analysis of regulation of the dnaA, dnaN, recF and gyrB genes of Escherichia coli. Gene 145: 17-24, 1994. PubMed: 8045420 |
| Shipped： | freeze-dried |