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购买须知
| 产品名称： | pGEM2Vk167 pSVk167 |
|---|---|
| 商品货号： | TS171553 |
| Designations： | pGEM2Vk167 pSVk167 |
| Species： | Mus musculus, mouse |
| Depositors： | U Storb |
| Applications： | The T7 promoter is used to make anti-sense RNA. |
| Vector： | Construct size (kb): 3.200000047683716 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.3100000023841858 Tissue: myeloma MOPC-167 cell line Gene product: immunoglobulin kappa chain V24 IgkV24 |
| Insert Size (kb)： | 0.310 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The EcoRI + BamHI double digest generates a 324 bp fragment by cutting within the polylinkers. Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--2.9, 0.3; BglI--3.2; BglII--uncut; HindIII--3.2. The antibodies encoded by myeloma MOPC-167 are specific to phosphorylcholine. The insert was cloned from p167kappa RI, and spans the leader--Vk167 region. The T7 promoter is used to make anti-sense RNA. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Storb U, et al. Trangenic mice with mu and kappa genes encoding antiphosphorylcholine antibodies. J. Exp. Med. 164: 627-641, 1986. PubMed: 2425036 Deanna Haasch, personal communication |