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| 产品名称： | pBJ-384 |
|---|---|
| 商品货号： | TS171558 |
| Designations： | pBJ-384 |
| Depositors： | C Hug |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.4000000953674320 Vector: pBJ-384 (phagemid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pGEX-3X, pBluescript KS II + Marker(s):ampR Construct size (kb): 4.400000095367432 Features: marker(s): ampR promoter for expression: GAL10...GAL1 promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 (phi10) replicon: f1 replicon: pMB1 MCS: BamHI...SacI MCS: KpnI...EcoRI coding sequence: GST |
| Applications： | contains easily purifiable cassette(s) for construction encodes removable tag for protein isolation vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4; EcoRI--4.4; SacI--4.4. The GAL10 RNA start site is disrupted, thus providing only one direction of transcription. Unless a start site for transcription is provided, little or no message should be made from inserts cloned into the GAL10 side of the promoter cassette. Do not use this side of the promoter unless you can insure that mRNA will be properly initiated by providing an RNA start site. The polylinker sites listed may not be unique in this vector. Plasmid containing a SacI-GST-GAL1/GAL10-XhoI cassette useful for making other constructions. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Christopher Hug, personal communication |
| Shipped： | freeze-dried |