| 产品名称: | 47z p47z |
|---|---|
| 商品货号: | TS171584 |
| Designations: | 47z p47z |
| Vector: | Construct size (kb): 5.699999809265137 |
| Insert: | DNA: genomic Insert lengths(kb): 2.299999952316284 Gene product: DNA Segment, single copy, homologous on X and Y DXYS5 Alleles: B1, A1, A2, B1, A1, A2, A1, B2, C1, C2, A1, A2, B2, C1, C2, A2 |
| Insert Size (kb): | 2.300 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--3.4, 2.4; EcoRI/BamHI--2.9, 2.3, 0.39; BamHI--5.7; PstI--5.7; PvuI--5.7. EcoRI detects a 2.6 kb band common to X and Y chromosomes. TaqI detects a 4.3 kb Y-specific band and X-specific bands of 3.2 and 1.5 kb. The HindIII/TaqI polymorphism has demonstrated co-dominant segregation in 8 families. The StuI polymorphism demonstrated Y-linked inheritance in 6 families. Wash in 2X SSC at 68C. |
| References: | Vergnaud G, et al. A deletion map of the human Y chromosome based on DNA hybridization. Am. J. Hum. Genet. 38: 109-124, 1986. PubMed: 3004206 Geldwerth D, et al. Extensive DNA sequence homologies between the human Y and the long arm of the X chromosome. EMBO J. 4: 1739-1743, 1985. PubMed: 4029125 Nakahori Y, et al. Two 47z DXYS5 RFLPs on the X and the Y chromosome. Nucleic Acids Res. 17: 2152, 1989. PubMed: 2564668 Jean Weissenbach, personal communication |