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| 产品名称： | p425 GALL |
|---|---|
| 商品货号： | TS171600 |
| Designations： | p425 GALL |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 7.5100002288818360 DESCRIPTION OF VECTOR: Intact vector size: 7.510 Type of vector: plasmid Cloning sites: SpeI BamHI SmaI PstI HindIII SalI XhoI Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI Construction: pRS425, GALL promoter Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): LEU2, -> terminator: CYC1, -> MCS: SpeI...XhoI, promoter for expression: GALL, marker(s): ampR, replicon: 2 micron, -> Vector: p425 GALL (plasmid) Promoters: Promoter for expression GALL Construction: pRS425, GALL promoter Marker(s):LEU2,ampR Construct size (kb): 7.510000228881836 Features: marker(s): LEU2 marker(s): ampR promoter for expression: GALL replicon: 2 micron MCS: SpeI...XhoI terminator: CYC1 |
| Applications： | YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.8; EcoRI--5.4, 2.3; SacI/XbaI--3.9, 3.2. The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose and is induced at a moderate level by galactose. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |