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微信小章| 产品名称： | Clostridioides difficile (Prevot) Lawson et al. |
|---|---|
| 商品货号： | TS171638 |
| Deposited As： | Clostridium difficile (Hall and OToole) Prevot |
| Strain Designations： | 3232 |
| Isolation： | Belgium Human feces (adult with diarrhea) |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Preceptrol®： | no |
| Type Strain： | no |
| Comments： | Nontoxigenic; Absence of tcdA and tcdB genes confirmed by PCR. Ref  Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303Binary toxin gene cdtB not amplified by PCR Toxinotype tcdA-, tcdB- Ribotype 010 |
| Medium： | ATCC® Medium 2107: Modified Reinforced Clostridial ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood |
| Growth Conditions： | Temperature: 37°C Atmosphere: Anaerobic |
| Name of Depositor： | E Tyler |
| References： | Delmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688 Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463 Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 |