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| 产品名称： | pS1 |
|---|---|
| 商品货号： | TS171651 |
| Designations： | pS1 |
| Depositors： | JJ McCormick |
| Biosafety Level： | 1 |
| Host： | Escherichia coli xa0HB101 |
| Vector Information： | Size (kb): 2.770
Vector: pS1 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s): ampR
Construct size (kb): 2.770
Features: insert detection: lacZamb1 marker(s): ampR promoter: lac replicon: pMB1 |
| Applications： | Expression vector
Vector permitting construction of fusion proteins
Vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuI--1.9, 0.9; EcoRI--2.8; HindIII--2.8; NotI--2.8; SspI--2.8. The plasmid contains the following restriction sites (bp from 0): DrdI--91, 992; KasI--233; PvuI--276, 2150; EcoRI--396; NotI--418; NsiI--493; HindIII--531; SapI--767; BsaI--1850; SspI--2585. Cloning vector with the multiple cloning site expanded by inserting an oligonucleotide at the SmaI site of pUC19, thereby generating an amber mutation in the lacZ coding sequence. The order of the major features in this plasmid is: KasI - 3 lacZamb1 - EcoRI/MCS/HindIII - 5 lacZamb1 - SapI - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Wang Q, et al. Mammalian expression vectors with modulatable promoters and two multiple cloning sites. Gene 119: 155-161, 1992. PubMed: 1327962 J J McCormick, personal communication |
| Shipped： | freeze-dried |