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| 产品名称： | pNB404 |
|---|---|
| 商品货号： | TS171667 |
| Designations： | pNB404 |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.0600004196167000 Vector: pNB404 (plasmid) Promoters: Promoter truncated CYC1 promoter (no UAS element) Construction: pBR322 Marker(s):URA3,ampR Construct size (kb): 10.0600004196167 Features: marker(s): URA3 marker(s): ampR promoter: truncated CYC1 promoter (no UAS element) replicon: 2 micron ori replicon: pMB1 coding sequence: lacZ |
| Applications： | YE-type (episomal) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 3.3, 2.3; XbaI--7.6, 3.8; SalI--11.0. This is one of three lacZ reporter plasmids that lack UAS promoter elements (ATCC 87509, 87510 & 87513). They differ by polylinker sites. DNA fragments can be inserted into these plasmids to assay UAS (upstream activation site) activity. The vector was constructed by Susan Henry. |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Bachhawat N, et al. Functional characterization of an inositol-sensitive upstream activation sequence in yeast. A cis-regulatory element responsible for inositol-choline mediated regulation of phospholipid biosynthesis. J. Biol. Chem. 270: 25087-25095, 1995. PubMed: 7559640 |
| Related Products： | component of:ATCC 87539 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid |