微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pBS-NC4.5 |
|---|---|
| 商品货号： | TS171680 |
| Designations： | pBS-NC4.5 |
| Species： | Tomato spotted wilt virus |
| Depositors： | TL German |
| Applications： | contains sequence |
| Vector： | Construct size (kb): 4.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript KS+ Intact vector size: 2.964 Type of vector: phagemid Vector end: PstI Vector end: PstI Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI Polylinker sites: SEE COMMENTS Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: tomato spotted wilt tospovirus Gene symbol: TSWV N Contains complete coding sequence?: Strain: Hawaiian L isolate Disease: tomato spotted wilt virus Type of DNA: cDNA Insert 5 end: PstI Insert 3 end: PstI Insert size (kb): 1.5 Cross references: Insert lengths(kb): 1.5 Tissue: tomato spotted wilt virus Disease: tomato spotted wilt virus Gene product: TSWV N |
| Insert Size (kb)： | 1.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.0, 1.5; PstI/PvuII--2.5, 0.8, 0.7, 0.3, 0.2; EcoRI--3.3, 1.2; BamHI--4.5. The 1.5 kb insert contains the cauliflower mosaic virus 35S RNA promoter and the nopaline synthase terminator. A PstI/PvuII digest yields an approximately 800 bp band containing the N gene ORF. |
| References： | Kim JW. Disease Resistance in Tobacco and Tomato Plants Transformed with the Tomato Spotted Wilt Virus Nucleocapsid Gene. Plant Dis. 78: 615-621, 1994. |