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| 产品名称： | cMHZ47 |
|---|---|
| 商品货号： | TS171812 |
| Designations： | cMHZ47 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Otherwise, RFLPs were observed under normal hybridization and wash stringencies. |
| Vector： | Construct size (kb): 22.5 |
| Insert： | DNA: genomic Insert lengths(kb): 15.0 Gene product: DNA Segment, single copy D13S52 |
| Insert Size (kb)： | 15.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--5.9 (doublet), 4.4, 3.5, 0.88; EcoRI--8.2, 6.6, 4.6, 3.1; TaqI--3.6, 2.0, 1.8, 1.5, 1.15 + smaller; BamHI--19.0, 3.2. Codominant segregation demonstrated in 50 3-generation families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The probe was pre-associated with excess human DNA before hybridization. Otherwise, RFLPs were observed under normal hybridization and wash stringencies. |
| References： | Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (cMHZ47) on chromosome 13 D13S52. Nucleic Acids Res. 16: 3119, 1988. PubMed: 2897112 |