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微信小章| 产品名称： | Plasmodium berghei Vincke and Lips |
|---|---|
| 商品货号： | TS171880 |
| Strain Designations： | NK65B |
| Application： | Vector borne research |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain Illinois, |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | Virulent clone. |
| Growth Conditions： | Duration: in vivo, mouse Protocol: ATCCNO: 50173 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject the entire thawed contents of the ampule intraperitoneally into a 6- to 9-week-old mouse. The strain should be monitored, especially after infecting with thawed material. To infect additional mice, draw a drop of tail blood into a 1.0 ml syringe containing 0.5 ml of sterile buffered anticoagulant, mix, and inoculate 0.1-0.2 ml of the suspension intraperitoneally/rat. |
| Subcultivation： | Protocol: ATCCNO: 50173 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject the entire thawed contents of the ampule intraperitoneally into a 6- to 9-week-old mouse. The strain should be monitored, especially after infecting with thawed material. To infect additional mice, draw a drop of tail blood into a 1.0 ml syringe containing 0.5 ml of sterile buffered anticoagulant, mix, and inoculate 0.1-0.2 ml of the suspension intraperitoneally/rat. |
| Cryopreservation： | CRYOPRESERVATION:xa0 Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust. 1.xa0xa0 To harvest parasites, inject host with ketamine (0.1x960.2 ml). 2.xa0xa0 Open chest cavity to expose heart and exsanguinate via cardiac puncture using Yaegers anticoagulant** (see below), 1 volume anticoagulant to 4 volumes blood.xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 Glass distilled HGlaxa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 3.xa0xa0 Centrifuge blood for 5 mins. at 1800 rpm in 50 ml centrifuge tube. 4.xa0xa0 Aspirate supernatant using sterile Pasteur pipet. 5.xa0xa0 Resuspend pellet gently in remaining supernatant. 6.xa0xa0 Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows: A.xa0 Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature. B.xa0 Add the remaining 4 volumes of glycerolyte and gently agitate. 7.xa0xa0 Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.). 8.xa0xa0 Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor. xa0xa0xa0xa0xa0xa0xa0 |
| Name of Depositor： | NE Alger |
| References： | Branton MBStudies of clonal populations of NK65 Plasmodium berghei Ph.D. thesis, Univ. Illinois, 1978 Alger NE, Keshavarz-Valian H. Plasmodium berghei: the effects of suppressor factor on vaccination. Int. J. Parasitol. 14: 301-307, 1984. PubMed: 6381347 derived from M. Yoeli strain NK65 (ATCC 30090) by mosquito passage, Urbana, IL, pre-1978 |