| 产品名称: | Chlamydophila pneumoniae (Grayston et al.) Everett et al. |
|---|---|
| 商品货号: | TS171909 |
| Classification: | Chlamydiaceae, Chlamydophila |
| Deposited As: | Chlamydia pneumoniae Grayston et al. |
| Agent: | Chlamydophila pneumoniae (Grayston et al.) Everett et al. |
| Strain: | AO3 |
| Applications: | Respiratory research |
| Positive for Mycoplasma: | Yes |
| Biosafety Level: | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format: | frozen 1 mL per vial |
| Storage Conditions: | -70°C or colder |
| Comments: | First coronary isolate of C. pneumoniae. FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth. xa0 |
| Effect on Host: | Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies. |
| Recommended Host: | HEp-2 cells (ATCC CCL-23) |
| Growth Conditions: | Temperature: 35°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent.
Incubation: 3 days, a 5% CO2 in air atmosphere is recommended |
| Name of Depositor: | JT Summersgill |
| Special Collection: | ATCC |
| Source: | Coronary atheroma of human with athcroslerosis |
| References: | Ramirez JA. Isolation of Chlamydia pneumoniae from the coronary artery of a patient with coronary atherosclerosis. The Chlamydia pneumoniae/Atherosclerosis Study Group. Ann. Intern. Med. 125: 979-982, 1996. PubMed: 8967709 This holding has tested positive for Mycoplasma contamination by PCR. |