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| 产品名称： | pcos6EMBL |
|---|---|
| 商品货号： | TS172049 |
| Designations： | pcos6EMBL |
| Depositors： | A Craig |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.00 Vector: pcos6EMBL (cosmid) Promoters: Promoter none Construction: pcos5EMBL, Pi Marker(s):kanR Construct size (kb): 6.0 Features: marker(s): kanR promoter: none replicon: R6K terminator: none enhancer: none |
| Applications： | vector for constructing cDNA libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. One of a family of cosmid vectors (ATCC 37568-37571) containing a mutated R6K origin for increased copy number. Has increased copy number over pcos5EMBL (ATCC 37570). |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Ehrich E, et al. A family of cosmid vectors with the multi-copy R6K replication origin. Gene 57: 229-237, 1987. PubMed: 2961651 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |