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| 产品名称： | pZZ2 |
|---|---|
| 商品货号： | TS172071 |
| Designations： | pZZ2 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | SJ Elledge |
| Applications： | mutation detection beta-galactosidase beta-D-galactosidase |
| Vector： | Construct size (kb): 11.5 |
| Insert： | DNA: genomic Insert lengths(kb): 0.8999999761581421 Gene product: ribonucleotide reductase (ribonucleoside-diphosphate reductase) large subunit(ribonucleotide reductase, large subunit) RNR3 Target Gene: beta-galactosidase |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--11.0, 0.9; EcoRI--12.0; SalI--12.0. An RNR3-lacZ transcriptional fusion construct wherein beta-galactosidase expression is regulated by the RNR3 promoter. Includes up to codon 61 of RNR3. Can be used to assay RNR3 activity, such as that induced by DNA damage. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Zhou Z, Elledge SJ. Isolation of crt mutants constitutive for transcription of the DNA damage inducible gene RNR3 in Saccharomyces cerevisiae. Genetics 131: 851-866, 1992. PubMed: 1516817 |