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| 产品名称： | pCu415CTR1-CYC1 |
|---|---|
| 商品货号： | TS172112 |
| Designations： | pCu415CTR1-CYC1 |
| Depositors： | S Labbe, DJ Thiele |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.6279997825622560 DESCRIPTION OF VECTOR: Intact vector size: 6.628 Type of vector: phagemid Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI Polylinker sites: SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI Xho Construction: pRS415, CTR1-CYC1 promoter Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): marker(s): LEU2, replicon: f1, terminator: CYC1, MCS: XhoI...SpeI, -> promoter for expression: CTR1-CYC1, -> replicon: pMB1 marker(s): ampR, centromere: CEN6 replicon: ARSH4 Vector: pCu415CTR1-CYC1 (phagemid) Promoters: Promoter for expression CTR1-CYC1 Construction: pRS415, CTR1-CYC1 promoter Marker(s):LEU2,ampR Construct size (kb): 6.627999782562256 Features: marker(s): LEU2 marker(s): ampR promoter for expression: CTR1-CYC1 replicon: ARSH4 replicon: f1 replicon: pMB1 MCS: XhoI...SpeI terminator: CYC1 centromere: CEN6 |
| Applications： | cloning vector |
| Comments： | Restriction digests of the vector give the following sizes (kb): SacI--6.6; SacI/SpeI--6.2, 0.4. CTR3 and CTR1 are the copper transporter promoters and are repressed by the presence of copper and highly activated under copper-starvation conditions. The time course of copper-starvation activation and copper repression of a reporter gene is rapid. |
| References： | Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452 |
| Related Products： | component of:ATCC 87778 |
| Shipped： | dried |
| Shipping Information： | Distributed: DNA supplied by depositor (frozen) (amount: 100 ng) |