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| 产品名称： | pBUT |
|---|---|
| 商品货号： | TS172125 |
| Designations： | pBUT |
| GenBank Number： | M16451 |
| Species： | Homo sapiens, human |
| Depositors： | MB Perryman |
| Vector： | Construct size (kb): 2.779999971389771 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.1840000003576279 Tissue: placenta Gene product: creatine kinase, brain(creatine kinase B) CKB Alleles: D1, D2, D1, D2 |
| Insert Size (kb)： | 0.184 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.0, 0.2; HindIII--2.9; PstI--2.9; BamHI--2.9; EcoRI/HindIII--2.7, 0.2. Constructed from the 184 bp HaeIII fragment (nt 1150 to nt 1335) in the 3 untranslated region which is specific to B creatine kinase and does not hybridize to M creatine kinase. Hybridizes to mRNA from primary cultures of rat cardiac myocytes which express B creatine kinase. EcoRI linkers were added during construction. |
| References： | Dionne FT, et al. Ncol RFLP in human brain creatine kinase gene (CKBB). Nucleic Acids Res. 19: 195, 1991. PubMed: 1672741 Villarreal-Levy G, et al. Human creatine kinase: isolation and sequence analysis of cDNA clones for the B subunit, development of subunit-specific clones and determination of gene copy number. Biochem. Biophys. Res. Commun. 144: 1116-1127, 1987. PubMed: 3034271 |