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| 产品名称： | pHPS9R |
|---|---|
| 商品货号： | TS172136 |
| Designations： | pHPS9R |
| Depositors： | S Bron |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.0999999046325680 Vector: pHPS9R (plasmid) Construction: pHP13-2 Marker(s):eryR Construct size (kb): 4.099999904632568 Features: marker(s): eryR replicon: pMB1, pTA1060 |
| Applications： | Plasmid was not verified by restriction analysis. Recommended for pHPS9 (ATCC 37816, 37817) to permit alpha-complementation and plasmid marker rescue transformation for transformation of competent cells with plasmid monomers. pHPS9R serves as the helper plasmid. |
| Comments： | Plasmid was not verified by restriction analysis. Strain was checked for the following phenotypes: showed growth on LB + erythromycin (150 mcg/ml), white colonies on Xgal + IPTG, white colonies on Xgal + IPTG + erythromycin (150 mcg/ml). Recommended for pHPS9 (ATCC 37816, 37817) to permit alpha-complementation and plasmid marker rescue transformation for transformation of competent cells with plasmid monomers. pHPS9R serves as the helper plasmid. Differs from pHPS9 (ATCC 37816, 37817) by partial removal of chloramphenicol resistance sequence (downstream of the NcoI site) and removal of the P59 promoter and sequences encoding the cat86::lacZalpha fusion. |
| Media： | ATCC® Medium 2305: 1065 plus erythromycin (150 mcg/ml) |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Haima P, et al. Novel plasmid marker rescue transformation system for molecular cloning in Bacillus subtilis enabling direct selection of recombinants. Mol. Gen. Genet. 223: 185-191, 1990. PubMed: 2123518 |
| Shipped： | freeze-dried |