| 产品名称: | pMCT35.1 MCT35.1, pMCT35 |
|---|---|
| 商品货号: | TS172246 |
| Designations: | pMCT35.1 MCT35.1, pMCT35 |
| Species: | Homo sapiens, human |
| Applications: | Codominant segregation was observed with the MspI RFLP in 55 three-generation families. RsaI also identifies a polymorphism, but this observation has not been verified by family studies. Otherwise, RFLPs were observed under normal hybridization and washing conditions. |
| Vector: | Construct size (kb): 5.199999809265137 |
| Insert: | DNA: genomic Insert lengths(kb): 2.400000095367432 Gene product: DNA Segment, single copy D17S31 Alleles: A1, A2 |
| Insert Size (kb): | 2.400 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.6, 2.4; EcoRI--5.0; HindIII--5.0; PstI--3.15, 1.65; BamHI--3.5, 1.65. Codominant segregation was observed with the MspI RFLP in 55 three-generation families. RsaI also identifies a polymorphism, but this observation has not been verified by family studies. This probe was pre-associated with excess human DNA prior to hybridization. Otherwise, RFLPs were observed under normal hybridization and washing conditions. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, PstI, PvuII and TaqI. |
| References: | Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence pMCT35.1 on chromosome 17p D17S31. Nucleic Acids Res. 16: 783, 1988. PubMed: 2893351 |