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| 产品名称： | REP82X |
|---|---|
| 商品货号： | TS172331 |
| Designations： | REP82X |
| Depositors： | SL Forsburg |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.5000000000000000 Vector: REP82X (phagemid) Promoters: Promoter for expression nmt1** (much weaker nmt1) Construction: pUC119 Marker(s):ampR,ura4+ Construct size (kb): 8.5 Features: marker(s): ampR marker(s): ura4+ promoter for expression: nmt1** (much weaker nmt1) replicon: ars1 replicon: pMB1, f1 MCS: Xho...SmaI |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--5.7, 1.7, 1.1; EcoRI--7.2, 1.3; XhoI--8.5. REP3X (ATCC 87603) and REP4X (ATCC 87604) contain full strength nmt1 promoter whereas REP41X (ATCC 87605) and REP42X (ATCC 87607) with medium strength (nmt1*) and REP81X (ATCC 87606) and REP82X (TS172331) with low strength (nmt1**) due to mutation in the TATA box by Basi et al. (Gene 123:131-136, 1993). REP81X (ATCC 87606) and REP82X (TS172331) are different in that REP81X contains the LEU2 marker and REP82X, ura4+. REP82X was constructed by deleting the ATG in the polylinker of the original REP82 plasmid. An XhoI linker was cloned into the blunted NdeI-BamHI cut REP82 plasmid, deleting SalI site. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516 Susan L Forsburg, personal communication |
| Related Products： | component of:ATCC 87685 |
| Shipped： | freeze-dried |