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| 产品名称： | pHSREM2 |
|---|---|
| 商品货号： | TS172403 |
| Designations： | pHSREM2 |
| Depositors： | DC Knipple |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.4000000953674320 Vector: pHSREM2 (plasmid) Promoters: Promoter T3 Construction: pBluescript KS-, hsp70 transcription module Marker(s):ampR Construct size (kb): 3.400000095367432 Features: marker(s): ampR promoter: hsp70, T3, T7 replicon: pMB1, M13 terminator: hsp70 |
| Applications： | contains easily purifiable cassette(s) for construction vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA |
| Comments： | A KpnI/SacI double digest releases the hsp70 transcription module, for subsequent ligation into other plasmids such as those containing Drosophila P elements. KpnI BglII NotI XhoI-promoter-MCS-terminator-SpeI XbaI NotI EagI BstXI SacII SacI. Restriction digests of the clone give the following sizes (kb): PstI--3.4; EcoRI--3.5; HindIII--3.5. The transcription module is flanked by restriction sites (facilitating its removal), T7 and T3 promoters (for in vitro RNA synthesis), and primers for Sanger sequencing. There is also an M13 origin of replication. One of a series (ATCC 37642-37645) of plasmid vectors for expressing genes under the control of the Drosophila hsp70 promoter and terminator. Major Drosophila hsp70 regulator elements are: 2 heat shock consensus elements (-85 to -72 & -62 to -49), a TATA box (-33 to -26) & transcription initiation site (+1) in the 5 segment; the termination triplet and polyA site in the 3 segment. The KpnI/SacI fragment of pHSREM1 (ATCC 37642) containing the hsp70 transcription module was ligated into KpnI/SacI digested Bluescript KS-. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Knipple DC, Marsella-Herrick P. Versatile plasmid vectors for the construction, analysis and heat-inducible expression of hybrid genes in eukaryotic cells. Nucleic Acids Res. 16: 7748, 1988. PubMed: 3137532 Douglas C Knipple, personal communication |
| Shipped： | freeze-dried |