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| 产品名称： | pTBQ7 cTBQ7 |
|---|---|
| 商品货号： | TS172428 |
| Designations： | pTBQ7 cTBQ7 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Codominant segregation was observed in 32 three generation families. RFLPS were observed after competitive hybridization with total human DNA. |
| Vector： | Construct size (kb): 4.599999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 1.899999976158142 Gene product: DNA Segment, single copy D10S28 |
| Insert Size (kb)： | 1.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.6, 1.9; EcoRI--4.6; HindIII--4.6; BamHI--4.6; HincII--uncut; SacI--4.6. This clone has demonstrated some instability. We recommend picking single colonies to identify the correct plasmid and amplifying with antibiotic selection. Codominant segregation was observed in 32 three generation families. RFLPS were observed after competitive hybridization with total human DNA. Insert is a 1.9 kb HaeIII fragment from cosmid cTBQ7 (isolated from somatic hybrid cell line 762-8A which included human chromosomes 10 and Y). |
| References： | Bragg T, et al. Isolation and mapping of a polymorphic DNA sequence (cTBQ7) on chromosome 10 D10S28. Nucleic Acids Res. 16: 11395, 1988. PubMed: 2462730 White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325 |