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| 产品名称： | pCMVST |
|---|---|
| 商品货号： | TS172509 |
| Designations： | pCMVST |
| GenBank Number： | M92042 |
| Species： | Rattus norvegicus, rat |
| Depositors： | P Berninsone, CB Hirschberg |
| Applications： | The 1.7kb EcoRI fragment (representing the 3 end of the CDS) from pCMVST can be used as a probe to detect mRNAs of 8.5, 7.0 and 4.2kb in rat liver tissue. |
| Vector： | Construct size (kb): 9.800000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 5.099999904632568 Tissue: liver Gene product: N-heparan sulfate sulfotransferase(heparan sulfate-N-deacetylase/N-sulfotransferase) Nhsst* |
| Insert Size (kb)： | 5.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.4, 2.4; EcoRI--4.8, 2.4, 1.6, 1.0; HindIII--5.1, 4.7. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--0.00, 2.42, 4.05; BglII--0.43, 2.16, KpnI--1.54, 2.58; BamHI--2.20. The 1.7kb EcoRI fragment (representing the 3 end of the CDS) from pCMVST can be used as a probe to detect mRNAs of 8.5, 7.0 and 4.2kb in rat liver tissue. |
| References： | Hashimoto Y, et al. Molecular cloning and expression of rat liver N-heparan sulfate sulfotransferase. J. Biol. Chem. 267: 15744-15750, 1992. PubMed: 1379236 |