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| 产品名称： | YIp5-KanR |
|---|---|
| 商品货号： | TS172531 |
| Designations： | YIp5-KanR |
| Depositors： | CA Reddy |
| Biosafety Level： | 1 |
| Host： | Shipped in: E. coli C600 |
| Vector Information： | Size (kb): 7.0999999046325680 Vector: YIp5-KanR (plasmid) Construction: kanR of pNG35 into PvuII site of YIp5 Marker(s):URA3,ampR,kanR,tetR Construct size (kb): 7.099999904632568 Features: marker(s): ampR, kanR, tetR, URA3 replicon: pMB1 |
| Applications： | YI-type (integrating) shuttle vector integrating vector shuttle vector |
| Comments： | Vector provides selection markers for transformation of Saccharomyces cerevisiae or other eukaryotes based on G418 resistance. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Rao TR, Reddy CA. A YIp5-kanR plasmid useful for isolating ars from yeast and other eukaryotes based on G418 resistance selection. Nucleic Acids Res. 14: 7504, 1986. PubMed: 3532035 Struhl K, et al. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc. Natl. Acad. Sci. USA 76: 1035-1039, 1979. PubMed: 375221 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried plasmid in E. coli |