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| 产品名称： | G14D |
|---|---|
| 商品货号： | TS172543 |
| Organism： | Ictalurus punctatus, channel catfish |
| Tissue： | peripheral blood |
| Cell Type： | lymphocyte T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Marion strain of channel catfish |
| Applications： | The cells constitutively produce interleukin-like factors. The G14D cell line developed in 1998 is an immortal homozygous T cell line spontaneously derived by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from a normal gynogenetic channel catfish. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | diploid |
| Images： |  |
| Derivation： | The G14D cell line developed in 1998 is an immortal homozygous T cell line spontaneously derived by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from a normal gynogenetic channel catfish.. The cells were, subsequently, cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells constitutively produce interleukin-like factors. |
| Receptor Expression： | T cell antigen receptor, expressed |
| Comments： | The G14D cell line developed in 1998 is an immortal homozygous T cell line spontaneously derived by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from a normal gynogenetic channel catfish.. The cells were, subsequently, cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells constitutively produce interleukin-like factors. |
| Complete Growth Medium： | A 1:1 mixture of Leibovitzs L-15 medium with 2 mM L-glutamine and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 10% double distilled water and 5% heat-inactivated catfish serum. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml. Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Fetal Bovine Serum supplented with 7.5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 27.0°C |
| Name of Depositor： | NW Miller |
| Deposited As： | Ictalurus punctatus |
| Year of Origin： | 1998 |
| References： | Hogan RJ, et al. Molecular and Immunologic Characterization of Gynogenetic Channel Catfish (Ictalurus punctatus). Mar. Biotechnol. 1: 317-327, 1999. PubMed: 10489406 |