微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pTF1 |
|---|---|
| 商品货号： | TS172591 |
| Designations： | pTF1 |
| Depositors： | A Portner |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.0429999828338620 Vector: pTF1 (phagemid) Promoters: Promoter T7 (phi10) Construction: pAR2529, pTZ18R, pBluescript KS+ Marker(s):ampR Construct size (kb): 3.042999982833862 Features: marker(s): ampR promoter: T7 (phi10) replicon: pMB1, f1 terminator: T phi |
| Applications： | expression vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.0; EcoRI--3.0; HindIII--3.0. High-copy-number plasmid (in bacteria) with increased efficiency of liposome-mediated introduction into cultured cells. Can express foreign sequences in cells producing T7 RNA polymerase, such as that encoded by recombinant vaccinia virus vTF7-3 ATCC VR-2153. A correction to the published sequence has been submitted. Sequence 2173-2252 should read: GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG. Constructed by inserting the 152 bp BamHI-BglII fragment from pAR2529 into the BamHI site of pTZ18R. Polylinker sites were removed and were replaced with the 48 bp EcoRI-KpnI fragment of pBluescript KS+. The order of the major features of the plasmid is: universal primer sequence - XbaI - T7 phi 10 terminator < - XbaI - BamHI/MCS/EcoRI - < T7 phi 10 promoter - reverse primer sequence - pMB1 ori - < ampR - f1 ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Takahashi T, et al. A plasmid that improves the efficiency of foreign gene expression by intracellular T7 RNA polymerase. Genet. Anal. Tech. Appl. 9: 91-95, 1992. PubMed: 1476796 |
| Shipped： | freeze-dried |