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| 产品名称： | pFRC11 pRC2 |
|---|---|
| 商品货号： | TS172595 |
| Designations： | pFRC11 pRC2 |
| GenBank Number： | J04357 |
| Species： | Red clover necrotic mosaic virus |
| Depositors： | Z Xiong |
| Applications： | The insert is oriented so that the transcript from the T7 promoter produces mRNA with positive polarity. |
| Vector： | Construct size (kb): 7.900000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.878000020980835 Gene product: 27 kDa protein( GROEL, heat shock 27 kDa protein 1, heat shock 27kD protein 1) |
| Insert Size (kb)： | 3.878 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.2, 2.3, 0.6, 0.55, 0.5, 0.3; SstI/SphI--4.2, 3.4; BamHI--4.6, 1.55, 1.4. pFRC11 includes the final nucleotide positions 3-3881. The 27 kDa ORF is encoded in sequences 123-831, the 57 kDa ORF from 926-2423, and the 37 kDa capsid ORF from 2427-3444. If there is a ribosomal frameshift, an 88 kDa fusion protein can be generated from 123-2423. The insert is oriented so that the transcript from the T7 promoter produces mRNA with positive polarity. |
| Classification： | Dianthovirus |
| References： | Xiong Z, Lommel SA. The complete nucleotide sequence and genome organization of red clover necrotic mosaic virus RNA-1. Virology 171: 543-554, 1989. PubMed: 2763465 Zhongguo Xiong, personal communication |