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| 产品名称： | LORIST2 |
|---|---|
| 商品货号： | TS172670 |
| Designations： | LORIST2 |
| Depositors： | TJ Gibson |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.5599999427795410 Vector: LORIST2 (cosmid) Promoters: Promoter T7 Marker(s):kanR,neoR Construct size (kb): 5.559999942779541 Features: marker(s): kanR, neoR promoter: SP6, T7 replicon: lambda terminator: trpA rrnC |
| Applications： | vector for constructing genomic libraries vector for other uses vector permitting RNA synthesis in vitro |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.6; HindIII--5.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Cosmid vector for genomic library construction. Insert-end-specific probes may be generated from T7 and SP6 promoters. Transcription terminators somewhat insulate the vector genes from insert transcription. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Gibson TJ, et al. Lorist2, a cosmid with transcriptional terminators insulating vector genes from interference by promoters within the insert: effect on DNA yield and cloned insert frequency. Gene 53: 275-281, 1987. PubMed: 3301536 |
| Shipped： | freeze-dried |