| 产品名称: | pHBP-1 |
|---|---|
| 商品货号: | TS172686 |
| Designations: | pHBP-1 |
| GenBank Number: | M13577 |
| Species: | Homo sapiens, human |
| Depositors: | RA Lazzarini |
| Vector: | Construct size (kb): 3.900000095367432 |
| Insert: | DNA: cDNA Insert lengths(kb): 1.213000059127808 Tissue: brain stem/cerebellum Gene product: myelin basic protein MBP Alleles: A2, C2, A1, B1, B2, C1 |
| Insert Size (kb): | 1.213 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.21; HindIII--2.8, 1.02; PstI--2.85, 0.95; SmaI--3.9; BamHI--3.9. Contains about 36 bp 5 untranslated sequence, MBP coding region (516 bp) and 661 bp of the 1603 bp 3 untranslated region. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. PvuII RFLP has demonstrated co-dominant inheritance in 1 informative family. BamHI RFLP has demonstrated co-dominant inheritance in 1 informative family. |
| References: | Kamholz J, et al. Identification of three forms of human myelin basic protein by cDNA cloning. Proc. Natl. Acad. Sci. USA 83: 4962-4966, 1986. PubMed: 2425357 Kamholz J, et al. The human myelin basic protein gene: chromosomal localization and RFLP analysis. Am. J. Hum. Genet. 40: 365-373, 1987. PubMed: 2437795 Barbara Weiffenbach, personal communication |