| 产品名称: | pOR3 |
|---|---|
| 商品货号: | TS172723 |
| Designations: | pOR3 |
| GenBank Number: | M11457 |
| Species: | Homo sapiens, human |
| Depositors: | P Chambon |
| Vector: | Construct size (kb): 5.599999904632568 |
| Insert: | DNA: cDNA Insert lengths(kb): 1.299999952316284 Tissue: breast adenocarcinoma MCF7 cell line Gene product: estrogen receptor ESR Alleles: s, b, A1, A2 |
| Insert Size (kb): | 1.300 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 1.35; BamHI--5.8; PstI--3.8, 1.04, 0.98; HindIII--5.3, 0.4; AvaI--3.0, 2.6. Extends approximately from nt 300 - 1600 of the published sequence. Contains sequences encoding the epitope recognized by MAb H226, believed to recognize the DNA-binding domain. This DNA-binding domain is in a 900 bp EcoRI/HindIII fragment and a 380 bp BalI fragment. Includes sequences homologous to v-erb-A (aa 185-251). |
| References: | Justice MJ, et al. A genetic linkage map of mouse chromosome 10: localization of eighteen molecular markers using a single interspecific backcross. Genetics 125: 855-866, 1990. PubMed: 1975791 Castagnoli A, et al. PvuII RFLP inside the human estrogen receptor gene. Nucleic Acids Res. 15: 866, 1987. PubMed: 2881267 Walter P, et al. Cloning of the human estrogen receptor cDNA. Proc. Natl. Acad. Sci. USA 82: 7889-7893, 1985. PubMed: 3865204 Green S, et al. Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A. Nature 320: 134-139, 1986. PubMed: 3754034 Kumar V, et al. Functional domains of the human estrogen receptor. Cell 51: 941-951, 1987. PubMed: 3690665 |