微信小陈
微信小章
产品简介
购买须知
| 产品名称： | p11-1-111 |
|---|---|
| 商品货号： | TS172798 |
| Designations： | p11-1-111 |
| Species： | Homo sapiens, human |
| Depositors： | M Litt |
| Vector： | Construct size (kb): 8.600000381469727 |
| Insert： | DNA: genomic Insert lengths(kb): 3.0 Gene product: DNA Segment, single copy D12S6 Alleles: A1, A1, A2, A2, C1, C2, D1, D2 |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): AvaI--6.5, 2.8; SalI--9.3; PstI--6.4, 1.2 (doublet); HindIII--5.8, 3.1; EcoRI--5.6, 3.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. This is one of 4 probes from cosmid 1-11 which reveals RFLPs at D12S6. Using all 4 probes, the PIC of the locus is 0.73. This polymorphism is also detected by BamHI and HindIII. Constructed by inserting Sau3A fragment of cosmid 1-11 into BamHI site. |
| References： | Buroker NE, et al. Four restriction length polymorphisms revealed by probes from a single cosmid map to human chromosome 12q. Hum. Genet. 72: 86-94, 1986. PubMed: 3002956 OConnell P, et al. A primary genetic linkage map for human chromosome 12. Genomics 1: 93-102, 1987. PubMed: 3478299 Michael Litt, personal communication |